Author: Steven Obenauf, Susan Finazzo
PDF | 108MB
| This laboratory manual for allied health or general microbiology has been written with the student in mind. The authors have used their years of teaching microbiology and microbiology laboratory at all levels to identify and relate the fundamental concepts that are important to the understanding of the science and students’ success in their future field.
They have included case studies to exemplify the relevance of the science and extensive visual imagery to help students understand and learn the content. Most importantly, the authors hope this manual will help students experience the thrill of bench science and share some of the enthusiasm they have for microbiology, a field of science that is dynamic, exciting and touches every aspect of your life. |
Table of contents :
Cover
Title
Copyright
About the Authors
Contents
Laboratory Overview: Introduction
Laboratory Safety, Equipment, and Materials
Tools of the Laboratory: Basic Techniques
1 Use of the Microscope
2 Aseptic Techniques
3 Isolation Streak Plate
Eukaryotic Cells and Microorganisms
4 Microbial Phototrophs: Algae and Cyanobacteria
5 Protozoa
6 Fungi
7 Bacterial Motility: Hanging Drop Method
Methods for Microscopic Analysis: Staining
8 Simple Staining and Smear Preparation
9 Negative Stain
10 Capsule Stain
11 Gram Stain
12 Acid-Fast Stain (Ziehl-Neelsen and Kinyoun Methods)
13 Endospore Stain (Schaeffer-Fulton Method)
Methods for Culturing and Diagnosing Microbes: Media
14 Blood Agar
15 Mannitol Salt Agar
16 Eosin Methylene Blue Agar
17 MacConkey Agar
Microbial Nutrition, Ecology, and Growth
18 Nutrients and Growth: Chemical Content of Media
19 Osmotic Pressure and Growth
20 Oxygen and Growth: Aerotolerance
21 Oxygen and Growth: Catalase
22 Oxygen and Growth: Oxidase
Food and Water Safety
23 Plate Count
Treatment and Control of Microbial Growth
24 Killing by Ultraviolet Light
25 Antimicrobial Drug Susceptibility Testing (Kirby-Bauer Method)
Microbial Genetics and Genetic Engineering
26 ELISA and Using Nucleotide BLAST (Dry Lab)
27 Analysis of DNA: Electrophoresis
28 Gene Transfer: Transformation
Infectious Diseases
29 Parasitic Protozoa
30 Parasitic Worms
31 Transmission: Vectors of Disease
32 Transmission: Epidemiology Activity
Diagnosing Infections
33 Gel Immunoprecipitation (Immunodiffusion)
34 Passive (Indirect) Agglutination
35 Rapid Immunoassays
36 Hemagglutinin Inhibition Assay
37 EnteroPluri-Test
Environmental Microbiology
38 Soil Microbiology
39 Nitrate Reduction
Hydrolytic Enzymes and Disease Mechanisms
40 DNase Test
41 Gelatin Hydrolysis
42 Starch Hydrolysis
43 Casein Hydrolysis
Biochemical Testing and Diagnosis of Disease
44 Phenol Red Sugar Broth
45 Triple-Sugar Iron Agar
46 Litmus Milk
47 IMViC (Indole, Methyl Red, Voges-Proskauer, Citrate) Reactions
48 Urease Test
Putting It All Together
49 “Known” Bacteria for Biochemical Testing
50 Identification of Unknown Bacteria
Index |
